Fast virtual histology using X-ray in-line phase tomography: application to the 3D anatomy of maize developing seeds

作者：Rousseau David^*; Widiez Thomas; Di Tommaso Sylvaine; Rositi Hugo; Adrien Jerome; Maire Eric; Langer Max; Olivier Cecile; Peyrin Francoise; Rogowsky Peter

来源：Plant Methods, 2015, 11(1): 55.

DOI：10.1186/s13007-015-0098-y

• X ray in-line phase tomography
• Image segmentation
• Virtual histology
• plant development
• maize seeds

Background: Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography or X-ray absorption tomography have been used with various success, depending on the size and physical properties of the biological material.
Results: X-ray in-line phase tomography was applied for the imaging of internal structures of maize seeds at early stages of development, when the cells are metabolically fully active and water is the main cell content. This 3D imaging technique with histology-like spatial resolution is demonstrated to reveal the anatomy of seed compartments with unequalled contrast by comparison with X-ray absorption tomography. An associated image processing pipeline allowed to quantitatively segment in 3D the four compartments of the seed (embryo, endosperm, nucellus and pericarp) from 7 to 21 days after pollination.
Conclusion: This work constitutes an innovative quantitative use of X-ray in-line phase tomography as a nondestructive fast method to perform virtual histology and extends the developmental stages accessible by this technique which had previously been applied in seed biology to more mature samples.

• 出版日期2015-12-18