MicroRNA-124 regulates osteoclast differentiation

作者：Lee Youngkyun^*; Kim Hyo Jeong; Park Cheol Kyu; Kim Yong Gun; Lee Heon Jin; Kim Jae Young; Kim Hong Hee

来源：Bone, 2013, 56(2): 383-389.

DOI：10.1016/j.bone.2013.07.007

• Osteoclast
• miR-124
• NFATc1
• Differentiation

Osteoclasts are specialized cells for bone-resorption originated from precursors of macrophage/monocyte lineage. The receptor activator of NF kappa B ligand (RANKL) initiates osteoclast differentiation, in which nuclear factor of activated T cell cytoplasmic 1 (NFATc1) plays a key role as a master transcription factor. In the present report, we show that microRNA-124 (miR-124) regulates osteoclastogenesis of mouse bone marrow macrophages (BMMs) by suppressing NFATc1 expression. On the other hand, synthetic inhibitor that binds specifically to miR-124 enhanced osteoclast differentiation and NFATc1 expression. The overexpression of a constitutively active form of NFATc1 prevented the inhibitory effect of miR-124 on osteoclastogenesis. Finally, miR-124 also affected the proliferation and motility of osteoclast precursors, the latter coinciding with the reduced expression of RhoA and Rac1. These findings not only reveal unprecedented role of miR-124 in osteoclastogenesis but also suggest a novel mode of regulation of NFATc1 in osteoclasts.

• 出版日期2013-10