Albumin Conjugating Amino Acid Schiff-Base Metal Complexes for Scavenging Superoxide Anion Radical

作者：Xu Zhili; Pan Sujuan; Li Gang; He Yu Feng; Wang Rong Min^*

来源：Journal of Inorganic and Organometallic Polymers and Materials, 2015, 25(6): 1313-1319.

DOI：10.1007/s10904-015-0242-1

• Superoxide anion radical
• Polymer antioxidant
• Schiff-base metal complexes
• Bovine serum albumin

Superoxide anion radical (O (2) (aEuro cent a') ) is a toxic reactive oxygen species (ROS). Schiff-base metal complexes have been widely studied as synthetic antioxidants to scavenge ROS. However, it is toxic and shows poor water solubility. In this work, a kind of novel water-soluble biopolymer/metal complex conjugate (HO-SAM@BSA) was prepared by binding the 4-hydroxy-salicylaldehyde amino acid Schiff-base metal complexes (HO-SAM, M = Cu, Zn, Co) with water-soluble biopolymer bovine serum albumin (BSA). The conjugates were characterized using IR, UV-Vis, circular dichroism spectra (CD), and polyacrylamide gel electrophoresis (PAGE). The results show that the structure of BSA is maintained when the binding rate (n(HO-SAM:) n(BSA)) of amino acid Schiff-base metal complexes is 10. In addition, the O (2) (aEuro cent a') scavenging activities of resultant conjugates were determined via nitrobule tetrazolium assay method. After combining HO-SAM into BSA, the poor water-solubility of HO-SAM is improved, and the O (2) (aEuro cent a') scavenging activity of BSA increases dramatically. The conjugation HO-SCCu@BSA displays excellent O (2) (aEuro cent a') scavenging activity. When the EC50 value was 0.10 mu mol/L, the analog quantity reached 41 % of natural SOD. Therefore, it can act as a bifunctional mimic of enzyme, which has a great application prospect in antioxidant drug.

• 出版日期2015-11
• 单位西北师范大学