Analysis of colonic autofluorescence spectra using multivariate curve resolution alternating least squares

作者：Liu, Lina; Yang, Changshun; Li, Weihua; Li, Buhong^*; Xie, Shusen

来源：Chinese Optics Letters, 2014, 12(5): 051705.

DOI：10.3788/COL201412.051705

• EXCITATION-EMISSION MATRICES
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• FLUORESCENCE

Autofluorescence (AF) spectra of colonic normal and adenocarcinoma tissues are measured under excitation of 337 nm and analyzed by multivariate curve resolution alternating least squares (MCR-ALS) method using non-negativity constraint. Collagen, nicotinamide adenine dinucleotide hydrate (NADH) and elastin are identified as the main contributing biomedical components. Fisher's discrminant anlysis (FDA) on the concentration profiles of the principle components (PCs) shows acceptable sensitivity, specificty and accuracy for discrminanting the adenocarcinoma tissues from the normal tissues. MCR-ALS is a powerful tool for characterzing the spectra profiles of the main biochemical components in neoplasm transformation.

• 出版日期2014-5
• 单位福建师范大学; 福建省立医院