An autonomous CMOS hysteretic sensor for the detection of desorption-free DNA hybridization

作者：Lee Kang Ho; Choi Suk Hwan; Lee Jeong Oen; Sohn Mi Jin; Yoon Jun Bo; Cho Gyu Hyeong^*

来源：Biosensors and Bioelectronics, 2011, 26(11): 4591-4595.

DOI：10.1016/j.bios.2011.04.033

• Capacitance
• DNA hybridization
• Desorption
• Impedance
• Sensor

This paper describes a sensor for label-free, fully electrical detection of DNA hybridization based on capacitive changes in the electrode-electrolyte interface. The sensor measures capacitive changes in real time according to a charging-discharging principle that is limited by the hysteresis In addition, a novel autonomous searching technique, which exclusively monitors desorption-free hybridized electrodes among electrode arrays, enhances the performance of the sensor compared with conventional capacitive measurement. The sensor system achieves a detection range of 80 dB. The integrated circuit sensor is fabricated with a 0.35 mu m CMOS process. The proposed sensor offers rapid, robust and inexpensive measurement of capacitance with highly integrated detection circuitry. It also facilitates quantitative evaluations of molecular densities on a chip with distinctive impedance variations by monitoring desorption-free hybridized electrodes. Our electrical biosensor has great potential for use with bio analytical tools and point-of-care diagnosis.

• 出版日期2011-7-15