An integrated microfluidic device for rapid serodiagnosis of amebiasis

作者：Zhao Wang; Zhang Li; Jing Wenwen; Liu Sixiu; Tachibana Hiroshi; Cheng Xunjia^*; Sui Guodong

来源：Biomicrofluidics, 2013, 7(1): 011101.

DOI：10.1063/1.4793222

• biomedical equipment
• bioMEMS
• diseases
• microfabrication
• microfluidics
• molecular biophysics
• patient diagnosis
• proteins

A microfluidic device was successfully fabricated for the rapid serodiagnosis of amebiasis. A micro bead-based immunoassay was fabricated within integrated microfluidic chip to detect the antibody to Entamoeba histolytica in serum samples. In this assay, a recombinant fragment of C terminus of intermediate subunit of galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica (C-Igl, aa 603-1088) has been utilized instead of the crude antigen. This device was validated with serum samples from patients with amebiasis and showed great sensitivity. The serodiagnosis can be completed within 20 min with 2 mu l sample consumption. The device can be applied for the rapid and cheap diagnosis of other infectious disease, especially for the developing countries with very limited medical facilities.

• 出版日期2013-1
• 单位复旦大学