Antioxidant and anticholinesterase potential of diterpenoid alkaloids from Aconitum heterophyllum

作者：Ahmad Hanif; Ahmad Shujaat; Shah Syed Adnan Ali; Latif Abdul; Ali Mumtaz; Khan Farman Ali; Tahir Muhammad Nawaz; Shaheen Farzana; Wadood Abdul; Ahmad Manzoor^*

来源：Bioorganic & Medicinal Chemistry, 2017, 25(13): 3368-3376.

DOI：10.1016/j.bmc.2017.04.022

• Aconitum heterophyllum
• Diterpenoid alkaloids
• X-ray crystallography
• Antioxidant activity
• Enzyme inhibition activity

Extensive chromatographic separations performed on the basic (pH = 8-10) chloroform soluble fraction of Aconitum heterophyllum resulted in the isolation of three new diterpenoid alkaloids, 6 beta-Methoxy, 9 beta-dihydroxylheteratisine (1), 1 alpha,11,13 beta-trihydroxylhetisine (2), 6,15 beta-dihydroxylhetisine (3), and the known compounds iso-atisine (4), heteratisine (5), hetisinone (6), 19-epi-isoatisine (7), and atidine (8). Structures of the isolated compounds were established by means of mass and NMR spectroscopy as well as single crystal X-ray crystallography. Compounds 1-8 were screened for their antioxidant and enzyme inhibition activities followed by in silico studies to find out the possible inhibitory mechanism of the tested compounds. This work is the first report demonstrating significant antioxidant and anti cholinesterase potentials of diterpenoid alkaloids isolated from a natural source.

• 出版日期2017-7-1