Desulfovibrio vulgaris Growth Coupled to Formate-Driven H-2 Production

作者：Martins Monica; Mourato Claudia; Pereira Ines A C^*

来源：Environmental Science & Technology, 2015, 49(24): 14655-14662.

DOI：10.1021/acs.est.5b02251

Formate is recognized as a superior substrate for biological H-2 production by several bacteria. However, the growth of a single organism coupled to this energetic pathway has not been shown in mesophilic conditions. In the present study, a bioreactor with gas sparging was used, where we observed for the first time that H-2 production from formate can be coupled with growth of the model sulfate-reducing bacterium Desulfovibrio vulgaris in the absence of sulfate or a syntrophic partner. In these conditions, D. vulgaris had a maximum growth rate of 0.078 h(-1) and a doubling time of 9 h, and the Delta G of the reaction ranged between -21 and -18 kJ mol(-1). This is the first report of a single mesophilic organism that can grow while catalyzing the oxidation of formate to H-2 and bicarbonate. Furthermore, high volumetric and specific H-2 production rates (125 mL L-1 and 2500 mL g(dcw) h(-1)) were achieved in a new bioreactor designed and optimized for H-2 production. This high H-2 production demonstrates that the nonconventional H-2-producing organism D. vulgaris is a good biocatalyst for converting formate to H-2 .

• 出版日期2015-12-15