Application of tissue microarrays to study the influence of dexamethasone on NF-κB expression of pancreas in rat with severe acute pancreatitis

作者：Zhang, Xi Ping^*; Zhang, Ling; Xu, Hong Miao; Xu, Yan Ping; Cheng, Qi Hui; Wang, Jian Mei; Shen, Hai Ping

来源：Digestive Diseases and Sciences, 2008, 53(2): 571-580.

DOI：10.1007/s10620-007-9867-4

• Severe acute pancreatitis (SAP)
• dexamethasone
• NF-kappa B
• inflammatory mediators
• pancreas
• tissue microarrays

To discuss the influence of dexamethasone on NF-kappa B expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation group. The groups were divided into the 3, 6 and 12 h group with 15 rats in each group. The survivals, pancreas pathological changes were observed 3, 6 and 12 h after operation. The changes in expression levels of NF-kappa B protein of pancreas tissue microarray were observed. The treatment group was significantly lower than the model group at 3 and 6 h (P < 0.05) and than the model group at 12 h in pancreas pathological scores (P < 0.01). The expression level of NF-kappa B protein of pancreas head of the treatment group was significantly less than that of the model group at 3 h (P < 0.01). The alleviation of pancreatic tissue injury by dexamethasone during SAP might be closely related to its role in inhibiting NF-kappa B expression and regulating cytokines. The advantages of tissue microarrays in pancreatitis pathological examination include time and energy savings, high efficiency and representative results.

• 出版日期2008-2
• 单位浙江大学; 杭州市第一人民医院; 山西医科大学; 浙江中医药大学