Assembly of Drosophila Centromeric Nucleosomes Requires CID Dimerization

作者：Zhang Weiguo; Colmenares Serafin U; Karpen Gary H^*

来源：Molecular Cell, 2012, 45(2): 263-269.

DOI：10.1016/j.molcel.2011.12.010

Centromeres are essential chromosomal regions required for kinetochore assembly and chromosome segregation. The composition and organization of centromeric nucleosomes containing the essential histone H3 variant CENP-A (CID in Drosophila) is a fundamental, unresolved issue. Using immunoprecipitation of CID mononucleosomes and cysteine crosslinking, we demonstrate that centromeric nucleosomes contain CID dimers in vivo. Furthermore, CID dimerization and centromeric targeting require a residue implicated in formation of the four-helix bundle, which mediates intranucleosomal H3 dimerization and nucleosome integrity. Taken together, our findings suggest that CID nucleosomes are octameric in vivo and that CID dimerization is essential for correct centromere assembly.

• 出版日期2012-1-27