A new naphthalene glycoside from Chimaphila umbellata inhibits the RANKL-stimulated osteoclast differentiation

作者：Shin Byong kyu; Kim Jinhee; Kang Ki Sung; Piao Hui Shan; Park Jeong Hill^*; Hwang Gwi Seo

来源：Archives of Pharmacal Research, 2015, 38(11): 2059-2065.

DOI：10.1007/s12272-015-0598-3

• Chimaphila umbellata
• 2,7-Dimethyl-1,4-dihydroxynaphthalene-1-O-beta-D-glucopyranoside
• Osteoclast
• Receptor activator of nuclear factor-kappa B ligand
• Tartrate-resistant acid phosphatase

A new naphthalene glycoside was isolated from the leaves and stems of Chimaphila umbellata Barton. Its chemical structure was elucidated to be 2,7-dimethyl-1,4-dihydroxynaphthalene-1-O-beta-d-glucopyranoside (DMDHNG), based on spectroscopic evidence. DMDHNG significantly inhibited the receptor activator of nuclear factor-kappa B ligand (RANKL)-induced tartrate-resistant acid phosphatase (TRAP) activity and the formation of multinucleated osteoclasts in a dose-dependent manner. In addition, the new glycoside inhibited the RANKL-induced mRNA expression of osteoclast-associated genes that encode TRAP, cathepsin K, and another transcription factor-nuclear factor of activated T-cells c1. We believe that the inhibitory effects of DMDHNG on the osteoclast differentiation may be exploited for a therapeutic benefit.

• 出版日期2015-11
• 单位延边大学