科研之友
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摘要
The adsorption of collagen on spin-coated poly(lactic acid) (PLA) surface was investigated using quartz crystal microbalance (QCM) The effects of concentration (0 similar to 9 25 mu g/mL) and temperature (10 similar to 50 degrees C) on adsorption were studied The results show that with the increasing concentration of collagen, the adsorption amount and initial adsorption rate on spin-coated PLA surface increases correspondingly Langmuir model and Freundlich model were applied for data fitting The isotherm equations q = 1169 0 99c/1 + 0 99c and q = 610c(1/3 79) were obtained It is found that Langmuir model described experiments better than the Freundlich model The kinetic experimental data were correlated with the Lagergren pseudo-first order kinetic model and the Lagergren pseudo-second order kinetic model It is shown that under low concentrations the Lagergren pseudo-first order kinetic model fits the results better and the Lagergren pseudo-second order kinetic fits the results better under high concentrations It means that the diffusion process is the rate-controlling step under low concentration and the interaction between collagen and PLA surface is the rate-controlling step under high concentrations The AFM Images show that the collagen adsorbed on PLA surface formed mesh-like structure The adsorption was significantly influenced by temperature changes in the adsorption buffer, indicating that the collagen is a kind of temperature-sensitive material The results also indicate that collagen denaturalizes gradually with increasing temperature in the range (10 similar to 40 degrees C) The adsorption quantity drops sharply when the temperature approaches (40 similar to 45 degrees C), 40 degrees C is the denaturizing temperature of collagen under present experimental conditions
- 出版日期2010-11-20
- 单位华东理工大学
