Antitumor activity and antitumor mechanism of triphenylphosphonium chitosan against liver carcinoma

作者：Huang, Haochao; Wu, Haiwei; Huang, Yongrui; Zhang, Shuangying; Lam, Yuetwai; Ao, Ningjian^*

来源：Journal of Materials Research, 2018, 33(17): 2586-2597.

DOI：10.1557/jmr.2018.255

• triphenylphosphonium chitosan
• antitumor activity and mechanism
• liver carcinoma

N-(3-Carboxypropyl) triphenylphosphonium bromide chitosan (TPPB-CS) was synthesized and characterized by FTIR, H-1 NMR spectrometer, and Zeta potential. TPPB-CS showed a selectivity-toxicity among cancer cell lines (MG-63 and HepG2 cells) and mouse embryonic fibroblast cells (NIH3T3 cells). A significant effect on inhibiting cell migration in HepG2 cells was observed in vitro, and TPPB-CS could effectively inhibit tumor growth in H22-bearing mice in vivo. Furthermore, the distribution of cell cycle, the level of reactive oxygen species (ROS), mitochondrial transmembrane potential (Delta psi(m)), the expression of tumor necrosis factor alpha (TNF-alpha), and vascular endothelial growth factor (VEGF) were examined to investigate the antitumor mechanism of TPPB-CS. The results suggested that the antitumor activity of TPPB-CS may be attributed to delay the cell cycle in S phase, alter the ROS and Delta psi(m) level, as well as regulate the TNF-alpha and VEGF secretion. TPPB-CS can become a promising anticancer drug for clinical therapy.

• 出版日期2018-9-14
• 单位暨南大学