Isolation and expression of an ecdysteroid-inducible neutral endopeptidase 24.11-like gene in wing discs of Bombyx mori

作者：Zhao X; Mita K; Shimada T; Okano K; Quan GX; Kanke E; Kawasaki H^*

来源：Insect Biochemistry and Molecular Biology, 2001, 31(12): 1213-1219.

DOI：10.1016/S0965-1748(01)00069-8

• Bombyx mori
• 20-hydroxyecdysone
• neutral endopeptidase
• wing disc
• metamorphosis

In the process of comparison of two cDNA libraries (W0, W2), we isolated a clone from the wing discs of Bombyx mori encoding a putative neutral endopeptidase 24.11-like gene. The predicted open reading frame encoded 772 amino acid residues, having about 53% identity with Drosophila GH07643, 36% with rat NEP, and 34% with rat ECE. This is the first NEP gene isolated in invertebrate. A 3.6-kb transcript was found to accumulate in the wing disc according to the increase of ecdysteroid titer during metamorphosis. Accumulation of the transcript was induced in wing discs with 20-hydroxyecdysone about 20h after incubation, which was inhibited by cycloheximide. This gene is ecdysone-inducible, appears to encode a functional protein, and may function during wing metamorphosis.

• 出版日期2001-11-1
• 单位RIKEN