Null EPAC mutants reveal a sequential order of versatile cAMP effects during Drosophila aversive odor learning

作者：Richlitzki Antje; Latour Philipp; Schwaerzel Martin

来源：Learning & Memory, 2017, 24(5): 210-215.

DOI：10.1101/lm.043646.116

Here, we define a role of the cAMP intermediate EPAC in Drosophila aversive odor learning by means of null epac mutants. Complementation analysis revealed that EPAC acts downstream from the rutabaga adenylyl cyclase and in parallel to protein kinase A. By means of targeted knockdown and genetic rescue we identified mushroom body Kenyon cells (KCs) as a necessary and sufficient site of EPAC action. We provide mechanistic insights by analyzing acquisition dynamics and using the "performance increment" as a means to access the trial-based sequential organization of odor learning. Thereby we show that versatile cAMP-dependent mechanisms are engaged within a sequential order that correlate to individual trials of the training session.

• 出版日期2017-5