Development and optimization of a method for the separation of platycosides in Platycodi Radix by comprehensive two-dimensional liquid chromatography with mass spectrometric detection

作者：Jeong Eun Kyung; Cha Hyun Jeong; Ha Young Wan; Kim Yeong Shik; Ha In Jin; Na Yun Cheol^*

来源：Journal of Chromatography A, 2010, 1217(26): 4375-4382.

DOI：10.1016/j.chroma.2010.04.053

• Comprehensive 2D-LC
• Platycoside
• Peak capacity
• Orthogonality

Comprehensive two-dimensional chromatography (LC x LC) using combinations of two columns (C-18 x CN and C-18 x NH2) was employed with electrospray (ESI) mass spectrometry to analyze platycosides from root extract. Based on the capability of the C-18, CN and NH2 columns to separate the platycosides, the orthogonality in two-dimensional space according to each combination of columns was predicted from the correlation coefficients between the retention times of the 17 compounds separated by the independent CN and C-18 columns, and NH2 and C-18 columns. The expected distribution of the peaks was also compared with the two-dimensional plots obtained by practical separation in an LC x LC system. The increased peak capacities using C-18 x NH2 allowed three minor components and five isomers of the platycosides to be newly separated, which were not identified with 1D-LC using the individual C-18 column, whereas the combination of C-18 x CN did not result in any improvement of the separation performance.

• 出版日期2010-6-25