Design and expression of a short peptide as an HIV detection probe

作者：Lines Jamie A; Yu Zhiqiang; Dedkova Larisa M; Chen Shengxi^*

来源：Biochemical and Biophysical Research Communications, 2014, 443(1): 308-312.

DOI：10.1016/j.bbrc.2013.11.095

• Fusion peptide
• In vivo expression
• gp120
• HIV detection
• Early stages

To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20-200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection. Published by Elsevier Inc.

• 出版日期2014-1-3