A hemocompatible polyurethane surface having dual fibrinolytic and nitric oxide generating functions

作者：Gu, Hao; Chen, Xianshuang; Liu, Xiaoli^*; Zhan, Wenjun; Lyu, Zhonglin; Yu, Qian; Wu, Zhaoqiang^*; Chen, Hong

来源：Journal of Materials Chemistry B, 2017, 5(5): 980-987.

DOI：10.1039/c6tb02735k

• LYSINE-DERIVATIZED POLYURETHANE
• SITU CATALYTIC GENERATION
• MUSCLE-CELL PROLIFERATION
• POLYMERIC COATINGS
• VASCULAR GRAFTS
• SIDE-CHAINS
• CLOT LYSIS
• IN-VITRO
• BLOOD
• ADSORPTION

Thrombus formation remains a serious problem in developing blood compatible materials. Despite continuous, intensive efforts over many years to prepare surfaces that prevent clotting, such surfaces have not been achieved; indeed it seems that surface-induced clotting is inevitable. An alternative approach is to accept that clotting will occur and to design surfaces so that small, nascent clots will be lysed before they can cause harm. The generation of plasmin, as in the fibrinolytic system, may be adopted for this purpose. The vascular endothelium (the inner surface of intact blood vessels) releases nitric oxide (NO) on a continuous basis. NO protects against platelet activation and aggregation, and also has an anti-proliferative effect on smooth muscle cells (SMCs). Based on these two important functions of the vascular system, the approach of constructing a fibrinolytic surface that generates NO is developed in the present work. Poly(oligo(ethylene glycol) methyl ether methacrylate-co-6-amino-2-(2methacylamido)- hexanoic acid) (poly(OEGMA-co-LysMA)) was attached to a vinyl-functionalized polyurethane (PU) surface by graft polymerization giving a surface (PU-POL) with protein-resistant properties (via poly(OEGMA)) and clot lysing properties (via poly(LysMA)). Selenocystamine, which catalyzes S-nitrosothiol decomposition to generate NO in the vasculature, was then immobilized on the PU-POL surface via covalent attachment. A dual functioning surface with fibrinolytic activity (lysis of nascent clots) and NO releasing ability (inhibition of platelet adhesion and SMC adhesion as well as proliferation) was thereby constructed.

• 出版日期2017-2-7
• 单位苏州大学