Apontic directly activates hedgehog and cyclin E for proper organ growth and patterning

作者：Wang, Xian-Feng; Shen, Yang; Cheng, Qian; Fu, Chong-Lei; Zhou, Zi-Zhang; Hirose, Susumu^*; Liu, Qing-Xin^*

来源：Scientific Reports, 2017, 7(1): 12470.

DOI：10.1038/s41598-017-12766-w

• DROSOPHILA EMBRYOGENESIS
• CUBITUS-INTERRUPTUS
• PERIPODIAL MEMBRANE
• CELL-PROLIFERATION
• IMAGINAL DISKS
• S-PHASE
• GENE
• PROTEIN
• CANCER
• TRANSCRIPTION

Hedgehog (Hh) signaling pathway and Cyclin E are key players in cell proliferation and organ development. Hyperactivation of hh and cyclin E has been linked to several types of cancer. However, coordination of the expression of hh and cyclin E was not well understood. Here we show that an evolutionarily conserved transcription factor Apontic (Apt) directly activates hh and cyclin E through its binding site in the promoter regions of hh and cyclin E. This Apt-dependent proper expression of hh and cyclin E is required for cell proliferation and development of the Drosophila wing. Furthermore, Fibrinogen silencer-binding protein (FSBP), a mammalian homolog of Apt, also positively regulates Sonic hh (Shh), Desert hh (Dhh), Cyclin E1 (CCNE1) and Cyclin E2 (CCNE2) in cultured human cells, suggesting evolutionary conservation of the mechanism. Apt-mediated expression of hh and cyclin E can direct proliferation of Hh-expressing cells and simultaneous growth, patterning and differentiation of Hh-recipient cells. The discovery of the simultaneous expression of Hh and principal cell-cycle regulator Cyclin E by Apt implicates insight into the mechanism by which deregulated hh and cyclin E promotes tumor formation.

• 出版日期2017-9-29
• 单位山东农业大学