Association of SNAREs and Calcium Channels with the Borders of Cytoskeletal Cages Organizes the Secretory Machinery in Chromaffin Cells

作者：Torregrosa Hetland Cristina J; Villanueva Jose; Lopez Font Inmaculada; Garcia Martinez Virginia; Gil Amparo; Gonzalez Velez Virginia; Segura Javier; Viniegra Salvador; Gutierrez Luis M^*

来源：Cellular and Molecular Neurobiology, 2010, 30(8): 1315-1319.

DOI：10.1007/s10571-010-9565-1

• Chromaffin cells
• SNAREs
• Calcium channels
• Cytoskeleton
• Exocytosis
• Active sites

In chromaffin cells, SNARE proteins, forming the basic exocytotic machinery are present in membrane clusters of 500-600 nm in diameter. These microdomains containing both SNAP-25 and syntaxin-1 are dynamic and the expression of altered forms of SNAREs modifies not only their motion but also the mobility of the associated granules. It is also clear that SNARE microdomain location defines the place for individual vesicle fusion and that the alteration of cluster dynamics affects the fusion process itself. Interestingly, these SNARE patches colocalize with the borders of F-actin cages forming the cytoskeletal cortical network, and these borders also contain clusters of L- and P/Q type calcium channels. The organization of the secretory machinery in association with the borders of cytoskeletal cages seems to be an effective way to promote fast coupling between calcium entry and catecholamine release as demonstrated with the use of mathematical secretory models.

• 出版日期2010-11