摘要
Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the flourescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in flourescence enhancement of BHb in a dose-dependent manner. Furthermore, UVI-induced flourencence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H2O2), a tye of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced flourescence enhancement. In contrast, green light irradiation does not lead to flourescence enhancement of BHb no matter whether H2O2 is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced flourescence enhancement of BHb.
- 出版日期2012-8-30
- 单位南开大学