Detecting Malaria Hotspots: A Comparison of Rapid Diagnostic Test, Microscopy, and Polymerase Chain Reaction

作者：Mogeni Polycarp^*; Williams Thomas N; Omedo Irene; Kimani Domtila; Ngoi Joyce M; Mwacharo Jedida; Morter Richard; Nyundo Christopher; Wambua Juliana; Nyangweso George; Kapulu Melissa; Fegan Gregory; Bejon Philip

来源：Journal of Infectious Diseases, 2017, 216(9): 1091-1098.

DOI：10.1093/infdis/jix321

• polymerase chain reaction
• microscopy
• rapid diagnostic test
• asymptomatic parasitemia
• stable hotspots

Background. Malaria control strategies need to respond to geographical hotspots of transmission. Detection of hotspots depends on the sensitivity of the diagnostic tool used.
Methods. We conducted cross-sectional surveys in 3 sites within Kilifi County, Kenya, that had variable transmission intensities. Rapid diagnostic test (RDT), microscopy, and polymerase chain reaction (PCR) were used to detect asymptomatic parasitemia, and hotspots were detected using the spatial scan statistic.
Results. Eight thousand five hundred eighty-one study participants were surveyed in 3 sites. There were statistically significant malaria hotspots by RDT, microscopy, and PCR for all sites except by microscopy in 1 low transmission site. Pooled data analysis of hotspots by PCR overlapped with hotspots by microscopy at a moderate setting but not at 2 lower transmission settings. However, variations in degree of overlap were noted when data were analyzed by year. Hotspots by RDT were predictive of PCR/microscopy at the moderate setting, but not at the 2 low transmission settings. We observed long-term stability of hotspots by PCR and microscopy but not RDT.
Conclusion. Malaria control programs may consider PCR testing to guide asymptomatic malaria hotspot detection once the prevalence of infection falls.

• 出版日期2017-11-1