Simultaneous analysis of fructose and creatinine in urine samples using GC-MS/MS and enzymatic methods

作者：Ferenczy Viktoria^*; Kotora Peter; Szabo Alexandra Hengerics; Blasko Jaroslav; Kubinec Robert; Behulova Darina; Ngo Manh Thang; Bierhanzl Vaclav Matej; Cabala Radomir

来源：Monatshefte fur Chemie, 2016, 147(8): 1343-1347.

DOI：10.1007/s00706-016-1770-2

• Gas chromatography
• Mass spectrometry
• Enzymatic analysis
• Silylation
• Children's urine samples

This work focuses on the simultaneous analysis of creatinine and fructose with the gas chromatographic tandem mass spectrometric (GC-MS/MS) method after silylation with hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide and the comparison of GC-MS/MS and enzyme-catalyzed photometric analysis of creatinine. The GC-MS/MS method allows the direct silylation of urine samples without sample pretreatment before derivatization. Due to this option, the analysis and diagnostics of inborn metabolic diseases are faster than in the case of a separate determination of both analytes.

• 出版日期2016-8