Detection of fungal DNA in lysis-centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients

作者：Trovato L; Betta P; Romeo M G; Oliveri S^*

来源：Clinical Microbiology and Infection, 2012, 18(3): E63-E65.

DOI：10.1111/j.1469-0691.2011.03731.x

• Candidiasis
• diagnosis
• lysis-centrifugation system
• NICU
• PCR

We report data concerning the detection of fungal DNA directly from lysiscentrifugation blood culture to assess its value in the detection of fungaemia in 86 of the 347 patients admitted to the neonatal intensive-care unit between January 2009 and December 2010. The sensitivity and specificity of the PCR were 87.5% and 98.5%, respectively, with a positive predictive value of 93.3% and a negative predictive value of 97.1%. Detection of fungal DNA directly from blood culture Isolator 1.5 microbial tubes, without prior cultivation, is a promising approach for the rapid detection of Candida spp. in neonates with suspected candidaemia.

• 出版日期2012-3