Adhesion and Proliferation Behavior of Retinal Pigment Epithelial Cells on Hesperidin/PLGA Films

作者：Lee So Jin; Kang Su Ji; Kim Hye Yun; Lee Jung Hwan; Kim Eun Young; Kwon Soon Yong; Chung Jin Wha^*; Joo Choun Ki; Khang Gilson

来源：Polymer-Korea, 2014, 38(1): 24-30.

DOI：10.7317/pk.2014.38.1.24

• retinal pigment epithelial cells
• hesperidin
• PLGA
• hesperidin/PLGA hybrid film

Retinal pigment epithelium (RPE) plays an important role in maintaining the visual function and the degeneration of the RPE causes several retinal degeneration disease. In order to fabricate the suitable carrier for RPE transplantation, the hybrid poly(lactide-co-glycolide) (PLGA) film with hesperidin was prepared. Hesperidin has an anti-inflammatory and antioxidant characteristics. ARPE-19 was seeded on hesperidin/PLGA film and then, cell proliferation was determined by the MU assay, and cell adhesion and cell morphology were confirmed by SEM. Also, RT-PCR was performed to confirm the expression of the specific genes, and AEC immunohistochemical staining was performed to determine the expression of RPE65. As a result, we confirmed that attachment, proliferation and phenotype maintenance of RPE cells were more excellent on hesperidin/PLGA film than PLGA film, thereby we were able to confirm the potential applications of hesperidin/PLGA film as tissue engineering carrier for regeneration of retina.

• 出版日期2014-1