Analysis of dextromethorphan and dextrorphan in decomposed skeletal tissues by microwave assisted extraction, microplate solid-phase extraction and gas chromatography- mass spectrometry (MAE-MPSPE-GCMS)

作者：Fraser Candice D; Cornthwaite Heather M; Watterson James H^*

来源：Drug Testing and Analysis, 2015, 7(8): 708-713.

DOI：10.1002/dta.1754

• forensic toxicology
• bone
• microwave assisted extraction
• dextromethorphan
• dextrorphan

Analysis of decomposed skeletal tissues for dextromethorphan (DXM) and dextrorphan (DXT) using microwave assisted extraction (MAE), microplate solid-phase extraction (MPSPE) and gas chromatography-mass spectrometry (GC-MS) is described. Rats (n=3) received 100mg/kg DXM (i.p.) and were euthanized by CO2 asphyxiation roughly 20min post-dose. Remains decomposed to skeleton outdoors and vertebral bones were recovered, cleaned, and pulverized. Pulverized bone underwent MAE using methanol as an extraction solvent in a closed microwave system, followed by MPSPE and GC-MS. Analyte stability under MAE conditions was assessed and found to be stable for at least 60min irradiation time. The majority (>90%) of each analyte was recovered after 15min. The MPSPE-GCMS method was fit to a quadratic response (R-2>0.99), over the concentration range 1010 000ngmL(-1), with coefficients of variation <20% in triplicate analysis. The MPSPE-GCMS method displayed a limit of detection of 10ngmL(-1) for both analytes. Following MAE for 60min (80 degrees C, 1200W), MPSPE-GCMS analysis of vertebral bone of DXM-exposed rats detected both analytes in all samples (DXM: 0.9-1.5 mu gg(-1); DXT: 0.5-1.8 mu gg(-1)).

• 出版日期2015-8