LOW PREVALENCE OF HIV IN HIGH-RISK SERONEGATIVE HOMOSEXUAL MEN EVIDENCED BY VIRUS CULTURE AND POLYMERASE CHAIN-REACTION

作者：GUPTA P^*; KINGSLEY L; ANDERSON R; HO M; ENRICO A; DING M; COTTRILL M; WOLINSKY S; RINALDO C

来源：AIDS, 1992, 6(2): 143-149.

DOI：10.1097/00002030-199202000-00001

• HIV
• SERONEGATIVE
• VIRUS CULTURE
• POLYMERASE CHAIN REACTION
• PCR

Objective: To assess the presence of covert HIV-1 infection.(~)Setting: High-risk seronegative homosexual men from the Pittsburgh portion of the Multicenter AIDS Cohort Study were examined for the presence of HIV-1 infection.
Patients, participants: Ten men (group 1) were examined prospectively for the presence of HIV-1 in their freshly-obtained peripheral blood mononuclear cells (PBMC). Furthermore, cryopreserved PBMC from 26 men (group 2) at their first visit (1984-1985) were examined retrospectively for the presence of HIV-1.
Main outcome measures: PBMC samples from groups 1 and 2 were examined for HIV-1 by polymerase chain reaction (PCR) using gag, env and strong-stop (long terminal repeat) specific primers. In addition, fresh PBMC samples from group 1 were examined for HIV-1 by virus culture.
Results: None of the 10 PBMC samples from group 1 were positive for virus culture and PCR. Only one of the 26 men from group 2 was positive for gag and strong-stop DNA sequences. This PCR-positive, seronegative subject was found to be negative for HIV-1 by PCR at follow-up visits up to 48 months later. None of 15 seronegative low-risk homosexual men and 12 seronegative heterosexual men were found to be PCR-positive for HIV-1. However, six HIV-1-seropositive men were positive by PCR for gag, env, and strong-stop HIV-1 DNA sequences.
Conclusions: These results suggest a low prevalence of covert HIV-1 infection in high-risk seronegative homosexual men in our geographic area.

• 出版日期1992-2
• 单位西北大学