A novel approach for addressing diseases not yielding to effective vaccination? Immunization by replication-competent controlled virus

作者：Voellmy Richard^*; Bloom David C; Vilaboa Nuria

来源：Expert Review of Vaccines, 2015, 14(5): 637-651.

DOI：10.1586/14760584.2015.1013941

• controlled replication
• heat-activated and ligand-activated gene switches
• HSV
• regulation of replication-essential genes
• replication-competent virus
• vaccination
• viral immunization agent

Vaccination involves inoculation of a subject with a disabled disease-causing microbe or parts thereof. While vaccination has been highly successful, we still lack sufficiently effective vaccines for important infectious diseases. We propose that a more complete immune response than that elicited from a vaccine may be obtained from immunization with a disease-causing virus modified to subject replication-essential genes to the control of a gene switch activated by non-lethal heat in the presence of a drug-like compound. Upon inoculation, strictly localized replication of the virus would be triggered by a heat dose administered to the inoculation site. Activated virus would transiently replicate with an efficiency approaching that of the disease-causing virus and express all viral antigens. It may also vector heterologous antigens or control co-infecting microbes.

• 出版日期2015-5