Promicromonospora thailandica sp nov., isolated from marine sediment

作者：Thawai Chitti^*; Kudo Takuji

来源：International Journal of Systematic and Evolutionary Microbiology, 2012, 62: 2140-2144.

DOI：10.1099/ijs.0.035972-0

A novel actinomycete, strain S7F-02(T), which produced primary branched hyphae and fragmented into V- and Y-shaped bacillary cells, was isolated from marine sediment collected in the Andaman Sea, Trang Province, Thailand. Lysine was found to be the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars of strain S7F-02(T) were ribose, arabinose and glucose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H-4). The predominant cellular fatty acids were anteiso-C-15:0 and iso-C-15:0. The G+C content of the genomic DNA of strain S7F-02(T) was 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S7F-02(T) should be classified in the genus Promicromonospora. This strain formed a close association with Promicromonospora citrea DSM 43110(T), with which it shared 99.2% 16S rRNA gene sequence similarity. DNA-DNA hybridization data together with physiological and biochemical properties showed that strain S7F-02(T) could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain S7F-02(T) is considered to represent a novel species of the genus Promicromonospora, for which the name Promicromonospora thailandica sp. nov. is proposed. The type strain is S7F-02(T) (=BCC 41922(T)=JCM 17130(T)).

• 出版日期2012-9