Determination of lymphoid cell fate is dependent on the expression status of the IL-7 receptor

作者：Purohit SJ; Stephan RP; Kim HG; Herrin BR; Gartland L; Klug CA^*

来源：The EMBO Journal, 2003, 22(20): 5511-5521.

DOI：10.1093/emboj/cdg522

• B cell commitment
• EBF
• IL-7 receptor
• Pax5
• T cell development

Signaling through the IL-7 receptor (IL-7R) is necessary for the development of the earliest B- and T-lineage cells. IL-7R is first expressed on common lymphoid progenitor cells and is not detected on primitive common myeloid progenitors. In this study, we show that enforced expression of IL-7R on multipotential stem cells does not influence lymphoid versus myeloid cell fate. T cell development was compatible with sustained IL-7R expression; however, we observed a near complete block in B cell development at the onset of B-lineage commitment. Unlike pre-proB cells from control animals, developmentally-arrested IL-7R(+)B220(+)CD19(-)NK1.1(-)Ly-6C(-) cells failed to express EBF and Pax5. These results suggest that transient downregulation of IL-7R signaling is a necessary event for induction of EBF and Pax5 expression and B-lymphocyte commitment.

• 出版日期2003-10-15