A Study of an Aroma Extraction Method and Evaluation of the Aroma Extract Contribution to the Palatability and Reinforcement Effect of Dried Bonito Using Mice

作者：Amitsuka Takahiko^*; Okamura Maya; Shibashi Hiroko; Yamamoto Naoto; Saito Tsukasa; Nammoku Takashi; Tsuzuki Satoshi; Inoue Kazuo; Fushiki Tohru

来源：Journal of Nutritional Science and Vitaminology, 2014, 60(5): 328-333.

DOI：10.3177/jnsv.60.328

• dried bonito
• difference in aroma
• extraction method
• palatability
• reinforcement effect

Japanese cuisine has provided satisfying meals by fully utilizing the characteristic aroma and taste of katsuodashi (dried bonito broth), though it is not rich in sugars or fats. Katsuodashi is a very basic and indispensable element in Japanese cuisine, and is a hot water extract of katsuobushi (dried bonito). It has been reported that a dextrin solution containing natural dried bonito broth has a significant reinforcement effect, and has been suggested that the olfactory stimulation is important for the reinforcement effect. We examined various source materials for broth and identified an optimal method of aroma extraction by two-bottle choice and conditioned place preference tests in mice. By two-bottle choice tests, a solution containing arabushi (a type of katsuobushi) aroma extract obtained by a super-critical CO2 extraction method showed a significantly high preference. The conditioned place preference test showed the dashi-taste solution with arabushi supercritical CO2 extract had a reinforcement effect. Our results suggest that the arabushi extract obtained by supercritical CO2 extraction contains components responsible for preference and reinforcement effects in mice; it could become conducive to making Japanese cuisine more satisfying and palatable.

• 出版日期2014-10