A rapid quantitative activity assay shows that the Vibrio cholerae colonization factor GbpA is an active lytic polysaccharide monooxygenase

作者：Loose Jennifer S M; For**erg Zarah; Fraaije Marco W; Eijsink Vincent G H; Vaaje Kolstad Gustav^*

来源：FEBS LETTERS, 2014, 588(18): 3435-3440.

DOI：10.1016/j.febslet.2014.07.036

• Lytic polysaccharide monooxygenase
• AA10
• AA9
• AA11
• GbpA
• Colonization factor
• Vibrio cholerae

The discovery of the copper-dependent lytic polysaccharide monooxygenases (LPMOs) has revealed new territory for chemical and biochemical analysis. These unique mononuclear copper enzymes are abundant, suggesting functional diversity beyond their established roles in the depolymerization of biomass polysaccharides. At the same time basic biochemical methods for characterizing LPMOs, such as activity assays are not well developed. Here we describe a method for quantification of C1-oxidized chitooligosaccharides (aldonic acids), and hence LPMO activity. The method was used to quantify the activity of a four-domain LPMO from Vibrio cholerae, GbpA, which is a virulence factor with no obvious role in biomass processing.

• 出版日期2014-9-17