Development of a radioimmunoassay for the measurement of Bisphenol A in biological samples

作者：Kaddar Nisrin; Bendridi Nadia; Harthe Catherine; de Ravel Marc Rolland; Bienvenu Anne Lise; Cuilleron Claude Yves; Mappus Elisabeth; Pugeat Michel; Dechaud Henri^*

来源：Analytica Chimica Acta, 2009, 645(1-2): 1-4.

DOI：10.1016/j.aca.2009.04.036

• Bisphenol A
• Radioimmunoassay
• Human exposure
• Xenoestrogens
• Endocrine disruptors

Bisphenol A (BPA) is widely used in the manufacturing of polycarbonate plastic food and drink packaging. Possessing a weak estrogenic activity, BPA is listed among a growing list of endocrine disrupting compounds.
In this study, a polyclonal anti-BPA antibody was obtained by immunization with BPA-monocarboxymethylether covalently linked to BSA. The antibody demonstrates negligible cross-reactivity with most analogous BPA phenolic structures, and no cross-reactivity with endogenous steroids. An extraction step with ethyl acetate minimized matrix effects and allowed the BPA measurement in plasma and other biological samples.
Recovery after loading test was 96 +/- 4% and dilution tests had a linear profile (r(2) > 0.93). The limit of detection of the BPA RIA was 0.08 mu g L(-1) with an IC50 of 1.25 mu g L(-1). The intra-and inter-assay coefficients of variation were 5.6 and 8.6%, respectively at a BPA concentration of 0.7 mu g L(-1) and 6.9 and 5.7% at a BPA concentration of 1.3 mu g L(-1). A significant correlation was found between the values obtained by the RIA and HPLC-MS (r(2)=0.92) or HPLC coupled to a fluorescence detector (r(2)=0.80).
In conclusion, we described a BPA-RIA that is a suitable tool for evaluating human exposure to BRA.

• 出版日期2009-7-10