Detecting UV-induced nucleic-acid damage

作者：El Yazbi Amira F; Loppnow Glen R^*

来源：TrAC-Trends in Analytical Chemistry, 2014, 61: 83-91.

DOI：10.1016/j.trac.2014.05.010

• Comet assay
• DNA damage
• Electrophoresis
• Fluorescence
• Hairpin probe
• Immunoassay
• Molecular beacon
• Nucleic acid
• Polymerase chain reaction
• Ultraviolet light

Nucleic acids (NAs) play a vital role in numerous biological processes. Absorption of ultraviolet (UV) light by NAs can result in DNA damage at the molecular level, leading to mutagenesis, carcinogenesis, and cell death. Thus, much effort has been focused on developing methods for detecting damage in NA. This article discusses the different methods used for detecting UV-induced NA damage and compares them regarding their selectivity and sensitivity to the damage induced in NAs and their advantages and limitations.

• 出版日期2014-10