Superpulsed Low-Level Laser Therapy Protects Skeletal Muscle of mdx Mice against Damage, Inflammation and Morphological Changes Delaying Dystrophy Progression

作者：Pinto Leal Junior Ernesto Cesar; de Almeida Patricia; Tomazoni Shaiane Silva; Camillo de Carvalho Paulo de Tarso; Brandao Lopes Martins Rodrigo Alvaro; Frigo Lucio; Joensen Jon; Johnson Mark I; Bjordal Jan Magnus

来源：PLos One, 2014, 9(3): e89453.

DOI：10.1371/journal.pone.0089453

Aim: To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.
Methods: Ten animals were rando mu ly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age.
Results: Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p = 0.0203) in animals treated with LLLT (864.70 U.l(-1), SEM 226.10) than placebo (1708.00 U.l(-1), SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-alpha (placebo-control = 0.51 mu g/mu l [SEM 0.12], - LLLT = 0.048 mu g/mu l [SEM 0.01]), IL-1 beta (placebo-control = 2.292 mu g/mu l [SEM 0.74], - LLLT = 0.12 mu g/mu l [SEM 0.03]), IL-6 (placebo-control = 3.946 mu g/mu l [SEM 0.98], - LLLT = 0.854 mu g/mu l [SEM 0.33]), IL-10 (placebo-control = 1.116 mu g/mu l [SEM 0.22], - LLLT = 0.352 mu g/mu l [SEM 0.15]), and COX-2 (placebo-control = 4.984 mu g/mu l [SEM 1.18], LLLT = 1.470 mu g/mu l [SEM 0.73]).
Conclusion: Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy.

• 出版日期2014-3-5