A novel fluorescence-quenching immunochromatographic sensor for detection of the heavy metal chromium

作者：Fu, QiangQiang; Tang, Yong^*; Shi, CongYing; Zhang, XiaoLi; Xiang, JunJian; Liu, Xi

来源：Biosensors and Bioelectronics, 2013, 49: 399-402.

DOI：10.1016/j.bios.2013.04.048

• Immunochromatographic sensor
• Fluorescence quenching
• Chromium
• Small analytes
• Competitive immunoreaction

A novel fluorescence quenching immunochromatographic sensor (ICS) was developed for detecting chromium (Cr3+) within 15 min utilizing the fluorescence quenching function of gold nanoparticles (Au-NPs). The sensor performed with a positive readout. When the low concentrations of Cr3+ samples were applied, detection signals of the test line (T line) were quenched, whereas when higher concentration Cr3+ samples (1.56 ng/mL) were applied, the detection signal of the T line appeared. The detection signal intensity of the T line increased with increasing concentrations of Cr3+ . The low detection limit of developed fluorescence quenching ICS was 1.56 ng/mL. The fluorescence quenching ICS has a linear range of detection of Cr3+ comprising between 6.25 ng/mL to 800 ng/mL. The recoveries of the fluorescence quenching ICS to detect Cr3+ in tap water ranged from 94.7% to 101.7%. This result indicated that the developed sensor gave higher sensitivity and reliable reproducibility. It could provide a general detection method for small analyte in water samples.

• 出版日期2013-11-15
• 单位暨南大学