Detection and kinetic analysis of Epinotia aporema granulovirus in its lepidopteran host by real-time PCR

作者：Arneodo J D^*; Koenig G A; Berretta M F; Di Rienzo J A; Taboga O; Sciocco Cap A

来源：Archives of Virology, 2012, 157(6): 1149-1153.

DOI：10.1007/s00705-012-1265-3

Epinotia aporema granulovirus (EpapGV) has attracted interest as a potential biocontrol agent of the soybean pest Epinotia aporema in Argentina. Studies on virus/host interactions conducted so far have lacked an accurate method to assess the progress of virus load during the infection process. The present paper reports the development of a real-time PCR for EpapGV and its application to describe viral kinetics following ingestion of two different virus doses by last-instar E. aporema larvae. Real-time PCR was shown to be a reliable method to detect and quantify the presence of EpapGV in the analyzed samples. The increase in virus titer (log) exhibited a sigmoidal pattern, with an exponential growth phase between 24 and 48 h postinfection for both initial doses tested.

• 出版日期2012-6