Artemin transiently increases iNOS expression in primary cultured trigeminal ganglion neurons

作者：Shang, Haiqiong; Wang, Yan; Chao, Xiuhua; Sun, Gaoying; Bai, Xiaohui; Xu, Lei; Han, Yuechen; Li, Jianfeng; Wang, Haibo; Fan, Zhaomin^*

来源：Neuroscience Letters, 2017, 660: 34-38.

DOI：10.1016/j.neulet.2017.09.016

• Artemin
• Trigeminal ganglion
• iNOS
• GFR alpha 3
• TUJ-1

Artemin, a member of the glial cell line-derived neurotrophic factor family, is an important cytokine and a critical participant in trigeminal pain disorders such as tongue pain and migraine. However, the mechanisms underlying artemin's activity are largely unknown. In the present study, we used primary cultured trigeminal ganglion neurons (TGNs) to determine the effect of artemin on the expression of the inducible form of nitric oxide synthase (iNOS), which is released in response to painful and inflammatory stimuli. Following artemin treatment, western blot analysis showed that the protein level of iNOS was transiently elevated after artemin treatment for 15 min (p < 0.05). Immunofluorescence revealed that both the expressions of iNOS and GFRa3 were significantly up-regulated after artemin treatment for 15 min. In addition, iNOS expression induced by artemin was co-localized with GFRa3 and TUJ-1 in primary cultured TGNs, respectively. Our results indicate a previously unknown role of artemin in regulating iNOS expression in primary cultured TGNs, and regulation of iNOS might be involved in the mechanism through which artemin participates in the trigeminal pain pathway.

• 出版日期2017-11-1
• 单位山东省立医院; 山东大学