Association between FOXP3 gene polymorphisms and risk of differentiated thyroid cancer in Chinese Han population

作者：Jiang, Weichao; Zheng, Lei; Xu, Lijuan; Zhang, Yang; Liu, Xingxin; Hu, Lihua^*; Wang, Xiaobei^*

来源：Journal of Clinical Laboratory Analysis, 2017, 31(5): e22104.

DOI：10.1002/jcla.22104

• differentiated thyroid cancer
• FOXP3
• single nucleotide polymorphism

BackgroundFoxp3 plays important roles in autoimmune and inflammatory diseases as well as human malignancies. This study aimed to investigate the association between Foxp3 gene polymorphisms and the susceptibility to differentiated thyroid cancers (DTC).
MethodsGenotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 350 DTC patients and 306 healthy controls. FOXP3 relative expression was measured by real-time quantitative PCR (qRT-PCR).
ResultsAA/AC genotype of Foxp3-rs3761548 was associated with a higher risk of DTC. The frequency of Foxp3-rs2280883 CC/CT genotype was lower in DTC patients. Besides, the AA/AC genotype of rs3761548 was more frequent in female DTC than male DTC. The association between two single nucleotide polymorphisms (SNPs) and clinical characteristics of DTC was further analyzed. We found that rs3761548 AA/AC genotype was more frequent in severe DTC patients (tumor diameter >1cm) compared with the relative tender DTC patients (tumor diameter <1cm). On the contrast, the frequency of rs2280883 CC/CT genotype was lower in severe DTC patients. In addition, the Foxp3 relative expression in DTC with AA/AC genotype of rs3761548 was higher than that of DTC with CC genotype.
ConclusionOur findings suggested that Foxp3 polymorphisms were associated with the risk of DTC in Chinese Han population.

• 出版日期2017-9
• 单位中国人民解放军总医院; 华中科技大学; 郑州大学