A novel near-infrared fluorescent probe for H2O2 in alkaline environment and the application for H2O2 imaging in vitro and in vivo

作者：Liu, Keyin; Shang, Huiming; Kong, Xiuqi; Ren, Mingguang; Wang, Jian-Yong; Liu, Yong; Lin, Weiying^*

来源：Biomaterials, 2016, 100: 162-171.

DOI：10.1016/j.biomaterials.2016.05.029

• Near-infrared fluorescence
• H2O2 probe
• Alkaline environment
• Cells
• In vivo imaging

H2O2 as one of the most important ROS (Reactive Oxygen Species) has more attack activity to bio-molecules such as DNA, RNA, protein and enzyme in alkaline environment and leads to a series of disease. However, no attention has been paid to the fluorescent detection of H2O2 in alkaline environment in the past. Herein, we reported the first ratiometric near-infrared fluorescent probe based on a boric acid derivative of Changsha near-infrared dye (CSBOH) for H2O2 detection in alkaline condition and the application for H2O2 imaging in vivo. ICT (intra-molecular charge transfer) mechanism was used in CSBOH to modulate the fluorescence change. The photophysical change of CSBOH was investigated by comparison with a phenol derivative of Changsha near-infrared dye (CSOH), a structural analogue bearing phenol group. In the presence of H2O2, CSBOH exhibited remarkably different fluorescence change at 650 nm and 720 nm when excited by 560 nm and 670 nm light respectively in alkaline buffer and showed high selectivity toward H2O2. Cellular experiments demonstrate that CSBOH can image endogenously generated H2O2 in macrophages and A431 cells. In vivo experiment demonstrates that both CSOH and CSBOH can be used for bio-imaging, and CSBOH can image H2O2 in living animal successfully.

• 出版日期2016-9
• 单位济南大学