BSA denaturation in the absence and the presence of urea studied by the iso-conversional method and the master plots method

作者：Cao, Xiaomin^*; Tian, Yun; Wang, Zhiyong; Liu, Yuwen; Wang, Cunxin

来源：Journal of Thermal Analysis and Calorimetry, 2010, 102(1): 75-81.

DOI：10.1007/s10973-010-0773-1

• BSA
• Urea
• DSC
• Denaturation
• The iso-conversional method and the master plots method

The kinetics of bovine serum albumin (BSA) denaturation in the absence and the presence of urea was studied by the iso-conversional method and the master plots method using differential scanning calorimetry (DSC). The observed denaturation process was irreversible and approximately conformed to the simple order reaction, and the denaturation did not follow rigorously first-order kinetic model or other integral order reaction models. The denaturation temperature (T (m)), apparent activation energy (E (a)), approximate order of reaction (n), and pre-exponential factor (A) all distinctly decreased as the 2 mol L-1 urea was added, which indicated that the urea accelerated the denaturation process of BSA and greatly reduced thermal and kinetic stability of BSA. This study also demonstrated that the iso-conversional method, in combination with the master plots method, provides a valuable and useful approach to the study of the kinetic process of protein denaturation.

• 出版日期2010-10
• 单位湖南省环境监测中心站; 武汉大学