A Subset of CXCR5(+) CD8(+) T Cells in the Germinal Centers From Human Tonsils and Lymph Nodes Help B Cells Produce Immunoglobulins

作者：Shen, Juan; Luo, Xi; Wu, Qiongli; Huang, Jun; Xiao, Guanying; Wang, Liantang; Yang, Binyan; Li, Huabin; Wu, Changyou^*

来源：Frontiers in Immunology, 2018, 9: 2287.

DOI：10.3389/fimmu.2018.02287

• CD8 T cell
• C-X-C chemokine receptor type 5
• Follicular
• B cell
• Tfh-like cell

Recent studies indicated that CXCR5(+)CD8(+) T cells in lymph nodes could eradicate virus-infected target cells. However, in the current study we found that a subset of CXCR5(+)CD8(+) T cells in the germinal centers from human tonsils or lymph nodes are predominately memory cells that express CD45RO and CD27. The involvement of CXCR5(+)CD8(+) T cells in humoral immune responses is suggested by their localization in B cell follicles and by the concomitant expression of costimulatory molecules, including CD40L and ICOS after activation. In addition, CXCR5(+)CD8(+) memory T cells produced significantly higher levels of IL-21, IFN-gamma, and IL-4 at mRNA and protein levels compared to CXCR5(-)CD8(+) memory T cells, but IL-21-expressing CXCR5(+)CD8(+) T cells did not express Granzyme B and perforin. When cocultured with sorted B cells, sorted CXCR5(+)CD8(+) T cells promoted the production of antibodies compared to sorted CXCR5(-)CD8(+) T cells. However, fixed CD8(+) T cells failed to help B cells and the neutralyzing antibodies against IL-21 or CD40L inhibited the promoting effects of sorted CXCR5(+)CD8(+) T cells on B cells for the production of antibodies. Finally, we found that in the germinal centers of lymph nodes from HIV-infected patients contained more CXCR5(+)CD8(+) T cells compared to normal lymph nodes. Due to their versatile functional capacities, CXCR5(+)CD8(+) T cells are promising candidate cells for immune therapies, particularly when CD4(+) T cell help are limited.

• 出版日期2018-10-5
• 单位中山大学; 广州医科大学; 广州市妇女儿童医疗中心