Atromentin-Induced Apoptosis in Human Leukemia U937 Cells

作者：Kim Jin Hee; Lee Choong Hwan^*

来源：Journal of Microbiology and Biotechnology, 2009, 19(9): 946-950.

DOI：10.4014/jmb.0811.617

• Apoptosis
• atromentin
• U937 cells

In the course of screening for apoptotic substances that induce apoptosis in human leukemia U937 cells, a fungal strain, F000487, which exhibits potent inducible activity, was selected. The active compound was purified from an ethyl acetate extract of the microorganism by Sep-pak C(18) column chromatography and HPLC, and was identified as atromentin by spectroscopic methods. This compound induced caspase-3 processing in human leukemia U937 cells. The caspase-3 and poly (ADP-ribose) polymerase (PARP) were induced by atromentin in a dose-dependent manner. Furthermore, DNA fragmentation was also induced by this compound in a dose-dependent manner. These results show that atromentin potently induces apoptosis in U937 cells and that atromentin-induced apoptosis is related to the selective activation of caspases.

• 出版日期2009-9