alpha(2A)-adrenoceptors, but not nitric oxide, mediate the peripheral cardiac sympatho-inhibition of moxonidine

作者：Cobos Puc Luis E^*; Aguayo Morales Hilda; Silva Belmares Yesenia; Gonzalez Zavala Maria A; Centurion David

来源：European Journal of Pharmacology, 2016, 782: 35-43.

DOI：10.1016/j.ejphr.2016.04.043

• alpha(2A)-adrenoceptor
• Heart rate
• I-1-Imidazoline receptor
• Moxonidine
• Nitric oxide

Moxonidine centrally inhibits the sympathetic activity through the I-1-imidazoline receptor and nitric oxide. In addition, inhibits the peripheral cardiac sympathetic outflow by alpha(2)-adrenoceptors/I-1-imidazoline receptors, although the role of alpha(2)-adrenoceptor subtypes or nitric oxide in the cardiac sympatho-inhibition induced by moxonidine are unknown. Therefore, the cardiac sympatho-inhibition induced by moxonidine (10 mu g/kg min) was evaluated before and after of the treatment with the following antagonists/inhibitor: (1) BRL 44408, (300 mu g/kg, alpha(2A)), imiloxan, (3000 mu g/kg, alpha(2B)), and JP-1302, (300 mu g/kg, alpha(2C)), in animals pretreated with AGN 192403 (3000 mu g/kg, I-1 antagonist); (2) N-omega-nitro-L-arginine methyl ester (L-NAME; 34, 100, and 340 mu g/kg min); and (3) the combinations of the highest dose of L-NAME plus AGN 192403 or BRL 44408. Additionally, the expression of the neuronal (nNOS) and inducible (iNOS) nitric oxide synthase in the stellate ganglion was determined after treatment with moxonidine (i.p. 0.56 mg/kg daily, during one week). The cardiac sympatho-inhibition of 10 mu g/kg min moxonidine was: (1) unaffected by imiloxan and JP-1302, under pretreatment with AGN 192403, or L-NAME (34, 100 and 340 mu g/kg min) given alone; (2) partially antagonized by the combination of 340 mu g/kg min L-NAME plus BRL 44408; and (3) abolished by BRL 44408 under treatment with AGN 192403. Furthermore, moxonidine did not modify the nNOS or iNOS protein expression in the stellate ganglion, the main source of postganglionic sympathetic neurons innervating the heart. In conclusion, our results suggest that the peripheral cardiac sympatho-inhibition induced by moxonidine is mediated by alpha(2A)-adrenoceptor subtype but not by nitric oxide.

• 出版日期2016-7-5