Antimycobacterial action of a new glycolipid-peptide complex obtained from extracellular metabolites of Raoultella ornithinolytica

作者：Fiolka Marta J^*; Grzywnowicz Krzysztof; Mendyk Ewaryst; Zagaja Miroslaw; Szewczyk Rafal; Rawski Michal; Keller Radoslaw; Rzymowska Jolanta; Wydrych Jerzy

来源：Acta Pathologica, Microbiologica et Immunologica Scandinavica, 2015, 123(12): 1069-1080.

DOI：10.1111/apm.12466

• Antimicrobial activity
• Mycobacterium smegmatis
• natural product
• bacterial metabolites

In this paper, an antimycobacterial component of extracellular metabolites of a gut bacterium Raoultella ornithinolytica from D.veneta earthworms was isolated and its antimycobacterial action was tested using Mycobacterium smegmatis. After incubation with the complex obtained, formation of pores and furrows in cell walls was observed using microscopic techniques. The cells lost their shape, stuck together and formed clusters. Surface-enhanced Raman spectroscopy analysis showed that, after incubation, the complex was attached to the cell walls of the Mycobacterium. Analyses of the component performed with Fourier transform infrared spectroscopy demonstrated high similarity to a bacteriocin nisin, but energy dispersive X-ray spectroscopy analysis revealed differences in the elemental composition of this antimicrobial peptide. The component with antimycobacterial activity was identified using mass spectrometry techniques as a glycolipid-peptide complex. As it exhibits no cytotoxicity on normal human fibroblasts, the glycolipid-peptide complex appears to be a promising compound for investigations of its activity against pathogenic mycobacteria.

• 出版日期2015-12