Molecular identification of scallop planktonic larvae using species-specific microsatellites

作者：Zhan Aibin; Hu Xiaoli; Bao Lisui; Lu Wei; Peng Wei; Wang Mingling; Hu Jingjie^*

来源：Acta Oceanologica Sinica, 2008, 27(5): 134-146.

• species identification
• scallop
• planktonic larva
• microsatellite
• hybridization

The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However, the larvae identification by morphological characteristics is notoriously difficult, mainly clue to the small size (usually being less than 150 W) and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species, the Zhikong scallop Chlamys farreri, the noble scallop C nobilis, the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers, which was specified by transferability analyses, assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1% similar to 2% larvae in mixed plankton samples. larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple, inexpensive and robust in identification analyses, and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years.

• 出版日期2008
• 单位中国海洋大学