科研之友
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摘要
Background: Formaldehyde (FA), one of the simplest organic molecules, is a flammable, pungent,irritating and colorless gas. This study aimed to investigate the effects of various concentrations ofFA vapor on sperm parameters and testicular tissue.Materials and Methods: In this experimental study, we randomly assigned 36 adult male miceto one control and two experimental groups (n=12 for each group). The control group (C) didnot receive FA. Group F1 (low concentration) was exposed to 10 ppm FA vapor and the F2 (highconcentration) group was exposed to 20 ppm FA vapor. FA was administered for ten days, eighthours per day for both groups. At the end of the exposure period, half of the animals in eachgroup were sacrificed 24 hours after exposure to detect any short-term effects; the rest of the micewere sacrificed 35 days later to assess for long-term effects. Sperm parameters were analyzed byComputer-assisted Sperm Analyzer (CASA) and histological changes determined. In addition, westudied changes in testosterone hormone. Data were analyzed by one-way ANOVA followed by theScheffe test using SPSS software.Results: Long-term effects of FA in the experimental groups included significant reductions insperm cell numbers and sperm viability. A drastic reduction in progressive motility and increasedabnormal sperm percentage (p%26lt;0.001) compared with the control group was also noted. Histologicalstudy of testes specimens in the experimental group revealed displacement of germinal cells, alongwith degeneration of Leydig cells and seminiferous tubules.Conclusion: Exposure to FA vapor can destroy testicular structure and decrease percentages ofconcentration, viability, normal morphology, and progressive motility, in addition to increasing thepercentage of immotile sperm.
- 出版日期2013
