科研之友
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摘要
Scutellariae Radix, the dried root of Scutellaria baicalensis Georgi, has been widely used in Asian countries for the treatment of dermatitis, diarrhoea, inflammatory disease and hepatic disease. A simple, sensitive and precise reversed-phase liquid chromatographic method with isocratic elution was developed to simultaneously determine four bioactive compounds in Scutellariae Radix: baicalein, baicalin, wogonin and wogonoside. Chromatographic analysis was performed on a YMC Pack Pro C-8 column (150 x 4.6mm(2), 3 mu m), with a mobile phase of 0.1% formic acid : acetonitrile (70 : 30, v/v) at a flow rate of 1.0mLmin(-1), and UV detection at 280 nm. Linear behaviour was observed over the investigated concentration range (0.25-10 mu gmL(-1)) for all analytes, with a correlation coefficient of %26gt;0.997. The intra-and inter-day precisions were %26lt;8.07%, and accuracies were 92.3-102.9%. This method was successfully applied for the analysis of marker compounds for the quality control of Scutellariae Radix extract.
- 出版日期2012
