科研之友
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摘要
Background: Heparanase activity plays a decisive role in biological processes associated with remodeling of the extracellular matrix (c g, cancer metastasis, angiogenesis, and in Heparanase gene overexpression has been associated with advanced stage and poor survival in several cancers We investigated the potential association between single nucleotide polymorphisms (SNPs) of the HPSE-1 gene. tumor susceptibility, clinicopathological parameters, and survival with gastric cancer among the Han population in northern China Methods: In this case control study, there were 155 patients with gastric cancer and 204 healthy controls The genotyping was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay Chi-square test was performed to exam differences of genotypes or alleles frequency between samples The effect of various variables on outcome was investigated by multivariate analysis using the Cox proportional hazards model Results: We identified four polymorphism in the HPSE-1 acne Polymorphisms in introns 2 and 3. exon8. and exon13 occurred at a minor allele frequency of >10% There was an increase in frequency of individuals with a genotype that carried the intron3 (A), exon8 (A). exon 13 (G) haplotype (AAG) in patients with gastric cancer compared with healthy individuals (P = 0 0001, OR = 7 467, 95% CI 2 274-24 509) SNP rs11099592 variant genotypes AG/AA were associated with a Borrmann type classification (P = 0 0115. OR = 0 182. 95% CI 0049-0 668) and invasion depth (P (1 020, OR = 0 34 I. 95% Cl 0 134-0 866). whereas SNP rs4328905 AG genotype was correlated to Lauren diffuse Evade = 0 027, OR = 0419. 95% CI I) 191-1917) SNP rs685690 I variant genotypes GC/CC were associated with a better tumor-related survival CP= 0028. OR = 0504. 95% CI 0 273-0 930) compared with GG genotype Conclusions: H PSE-1 polymo
- 出版日期2010-7-1
- 单位中国医科大学
