A microfluidic method for the selection of undifferentiated human embryonic stem cells and in situ analysis

作者：Jabart E; Rangarajan S; Lieu C; Hack J; Conboy I; Sohn L L^*

来源：Microfluidics and Nanofluidics, 2015, 18(5-6): 955-966.

DOI：10.1007/s10404-014-1485-9

• Human embryonic stem cells (hESCs)
• Cell sorting
• Label-free
• In situ analysis

Conventional cell-sorting methods such as fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS) can suffer from certain shortcomings such as lengthy sample preparation time, cell modification through antibody labeling, and cell damage due to exposure to high shear forces or to attachment of superparamagnetic Microbeads. In light of these drawbacks, we have recently developed a label-free, microfluidic platform that can not only select cells with minimal sample preparation but also enable analysis of cells in situ. We demonstrate the utility of our platform by successfully isolating undifferentiated human embryonic stem cells (hESCs) from a heterogeneous population based on the undifferentiated stem-cell marker SSEA-4. Importantly, we show that, in contrast to MACS or FACS, cells isolated by our method have very high viability (similar to 90 %). Overall, our platform technology could likely be applied to other cell types beyond hESCs and to a variety of heterogeneous cell populations in order to select and analyze cells of interest.

• 出版日期2015-5